氨基酸柱采用ODS键合硅胶为填充剂,填料采用高度可控的单分子层形成和封尾技术制备得到。通过柱前衍生化方法,18种氨基酸在柱上可获得优异的分离。
Column: |
Sepax AA, 4.6×250 mm |
Mobile phase: |
A: ACN : 0.1 M NaAc = 7 : 93 (v/v), pH=6.5; |
Time (min): |
0 15 18 25 30 30.01 45 |
Flow rate: |
1.0 mL/min |
Temperature: |
36 oC |
Detection: |
UV 254nm |
Injection Volume: |
5 µL |
Sample: |
18 Amino acids |
1.Asp | 2.Glu | 3.Ser | 4.Gly | 5.His | 6.Arg |
7.Thr | 8.Ala | 9.Pro | 10.Tyr | 11.Val | 12.Met |
13.Cys | 14.Ile | 15.Leu | 16.Phe | 17.Trp | 18.Lys |